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. 2016 Feb 25;6:22086. doi: 10.1038/srep22086

Figure 4. Neurite arborisation of cortical neurons isolated from adult Mth1/Ogg1-DKO but not wild-type mice was significantly impaired in the absence of antioxidants.

Figure 4

(a) Adult cortical neurons isolated from Mth1/Ogg1-DKO (TO-DKO) and wild-type mice were cultured for 5 days in the absence (−AO) or presence (+AO) of antioxidants and were subjected to MAP2-immunofluorescence microscopy. Representative images are shown, indicating a simpler neuritic branching pattern in neurons from TO-DKO mice cultured in the absence of antioxidants. Scale bar = 20 μm. (b) Sholl’s concentric sphere analysis shows severely impaired neurite arborisation in TO-DKO neurons in the absence of antioxidants. More than 16 neurons were examined in each culture condition. Combined data from two independent experiments are shown. Error bar = SEM. MANOVA, between subjects: F(3, 171) = 19.671, p < 0.0001; within subjects: interaction, p < 0.0001 (Univar G-G, ε = 0.311). TO-DKO (−AO) vs. other three groups, *p < 0.0001.