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. 2016 Feb 25;6:22086. doi: 10.1038/srep22086

Figure 5. Mth1/Ogg1-DKO neurons do not exhibit increased cell death in the absence of antioxidants.

Figure 5

(a) Adult cortical neurons isolated from adult Mth1/Ogg1-DKO (TO-DKO) and wild-type mice were cultured for 2 days in vitro (2DIV, left panels) and 5 days in vitro (5DIV, right panels) in the absence (−AO) or presence (+AO) of antioxidants in the B27 supplements, and were subjected to Hoechst 33258 (blue, nuclear staining) and propidium iodide (PI, red, dead cells) staining. Representative images are shown. (b) Viability. The percentage of PI-negative cells among Hoechst 33258-positive cells was determined. 2DIV (left, 2-way ANOVA, F(3, 16) = 0.7804, p = 0.522), and 5DIV (right, 2-way ANOVA, F(3, 16) = 0.1565, p = 0.924). Error bar = SEM. More than 11 cells in a given field were counted and five different fields per group (>79 cells/group) were examined. Scale bar = 40 μm.