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. 2016 Feb 25;6:22086. doi: 10.1038/srep22086

Figure 7. MTH1/OGG1 deficiencies cause mitochondrial dysfunction in adult cortical neurons.

Figure 7

(a) Adult cortical neurons isolated from Mth1/Ogg1-DKO (TO-DKO) and wild-type brains were cultured for 2 days in the absence (−AO) or presence (+AO) of antioxidants and were then exposed to JC-1 dye. JC-1 dye emits green-fluorescence in its monomer form with lower membrane potential, and red-fluorescence in its aggregate form in normal mitochondria. Scale bar = 20 μm. (b) Red/green ratio of JC-1 fluorescence in cell body (soma in a) and neurites. Mitochondrial membrane potentials were significantly decreased in TO-DKO neurons cultured without antioxidants. 2-way ANOVA, F(3, 80) = 7.495, p = 0.0002 *Tukey-HSD test, TO-DKO (−AO) vs. other three groups. p < 0.002. More than 17 neurons were examined for each condition. Error bar = SEM.