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. 2016 Feb 25;6:22086. doi: 10.1038/srep22086

Figure 8. MTH1/OGG1-defciencies significantly increased mitochondrial production of superoxide in adult cortical neurons cultured in the absence of antioxidants.

Figure 8

(a) Adult cortical neurons isolated from Mth1/Ogg1-DKO (TO-DKO) and wild-type brains were cultured for 2 days in the absence (−AO) or presence (+AO) of antioxidants, then incubated with MitoTracker (green) and MitoSOX (red). Representative images are shown. Scale bar = 20 μm. (b) MitoSOX index in cell body and neurites are shown as whisker-box plots. Mitochondrial superoxide production significantly increased in TO-DKO neurons cultured in the absence of antioxidants. More than 17 neurons were examined for each condition. Outliers are shown as dots. Wilcoxon/Kruskal–Wallis tests, chi square test p < 0.0001; post hoc comparison with Wilcoxon method, *p < 0.05, **p < 0.005, ***p < 0.0001.