Table 1. Comparison of the OICF method developed herein, on-chip PEG fusion, and two traditional benchtop methods (PEG and electrofusion) on the cell pairing rate and fusion efficiency.
| Cell pairing rate (%) Paired cells/all microstructure | Fusion efficiency (%) Hybrids/paired cells | Overall efficiency (%) Hybrids/all microstructures | |
|---|---|---|---|
| OICF on chip (HeLa & A549) | 57.14 ± 7.23 | 87.28 ± 6.94* | 50.08 ± 9.42 |
| PEG on chip | 53.08 ± 3.91 | 44.13 ± 10.21* | 23.59 ± 6.64 |
| PEG (bench)26 | Not applicable | <10 | <10 |
| Electro fusion (bench)27 | Not applicable | <20 | <20 |
*p < 0.01, (two-tailed t-test).