Table 1. % high spin upon substrate binding and relative rate of NADH consumption vs. WT (%) for mutants of CYP101 described here.

	% high spin (camphor/adamantanone)	NADH consumption rate (%), camphor	NADH consumption rate (%), adamantanone
WT (C334A)	98%/90%	(100)	66.9 ± 3.8
I160L	35%/0%	87.9 ± 3.4	52.3 ± 5.4
I160L/L166A	70%/60%	69.1 ± 1.3	34.6 ± 7.4
L166A	100%/90%	69.5 ± 2.2	18.1 ± 2.5
L166A/L244A	50%/10%	50.5 ± 4	36.3 ± 4.3
L244A	35%/0%	57.1 ± 2.6	52.0 ± 1.0
F98Y	20%/10%	69.9 ± 3	28.8 ± 1.1
N59G	0%/0%	19.9 ± 1.1	7.2 ± 2.5
G326A	90%/78%	63.6 ± 10.2	62.5 ± 15.6

All mutations are in the C334A background. See text for details.

Data for G326A is from ref. 16.