Table 2. Relative turnover efficiency (fractional coupling) of CYP101 mutants relative to WT (%).
camphor | adamantanone | |
---|---|---|
WT (C334A) | (100) | 62.9 ± 0.9 |
I160L | 95.7 ± 7 | 60.0 ± 1.5 |
I160L/L166A | 94.6 ± 0.5 | 49.7 ± 1.9 |
L166A | 92.0 ± 3.5 | 36.5 ± 2.1 |
L166A/L244A | 92.8 ± 2.6 | 7.0 ± 0.9 |
L244A | 90.2 ± 3.7 | 11.7 ± 1.7 |
F98Y | 96.2 ± 3.4 | 62.5 ± 1.2 |
N59G | 3.5 ± 0.8 | 2.7 ± 0.8 |
G326A | 66.6 ± 15.0 | 57.8 ± 4.0 |
Data for G326A is from ref. 16.