Table 1.
Maize | Cd stress (mg kg−1) | Inoculation | Cd concentrations in each cell fraction (mg kg−1) |
|||
---|---|---|---|---|---|---|
Cell wall | Chloroplast-leaf/trophoplast-root | Membrane and organelle | Soluble | |||
Root | 10 | −DSE | 65.77 ± 1.16 | 22.12 ± 1.05 | 7.57 ± 0.55 | 58.35 ± 3.03 |
+DSE | 34.33 ± 0.95*** | 4.62 ± 0.46*** | 1.65 ± 0.39*** | 25.58 ± 1.10*** | ||
50 | −DSE | 212.98 ± 6.60 | 120.97 ± 1.83 | 33.78 ± 0.81 | 234.45 ± 4.35 | |
+DSE | 126.45 ± 3.03*** | 25.60 ± 1.98*** | 13.48 ± 1.27*** | 83.67 ± 0.52*** | ||
100 | −DSE | 839.63 ± 4.87 | 363.06 ± 9.04 | 245.82 ± 13.09 | 809.57 ± 12.71 | |
+DSE | 679.56 ± 9.59*** | 98.33 ± 6.14*** | 72.77 ± 1.90*** | 367.15 ± 9.31*** | ||
Leaf | 10 | −DSE | 21.19 ± 0.01 | 11.65 ± 0.85 | 0.73 ± 0.03 | 25.13 ± 2.13 |
+DSE | 17.63 ± 0.88** | 6.18 ± 0.95** | 0.63 ± 0.03* | 11.68±1.83** | ||
50 | −DSE | 70.78 ± 3.05 | 36.33 ± 1.10 | 9.10 ± 0.53 | 76.55 ± 2.20 | |
+DSE | 66.88 ± 2.03 | 23.38 ± 1.12*** | 5.85 ± 0.44** | 32.28 ± 1.23*** | ||
100 | −DSE | 268.68 ± 5.60 | 156.40 ± 2.85 | 63.20 ± 0.35 | 206.95 ± 8.41 | |
+DSE | 153.68 ± 2.78*** | 74.72 ± 1.30*** | 18.67 ± 1.10*** | 54.37 ± 2.82*** |
Subcellular distribution of Cd in maize inoculated with DSE (+DSE) or not (−DSE) under a series of Cd2+ stresses (0, 10, 50, 100 mg kg−1), examining the effect of DSE inoculation (mg kg−1, means ± SEM, n = 6; *indicates significant differences at 0.01 < p < 0.05, **indicates significant differences at 0.001 < p < 0.01, ***indicates significant differences at p < 0.001 level, ANOVA). No Cd2+ was observed for the no Cd control (data not shown).