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. 2016 Feb 25;6:22041. doi: 10.1038/srep22041

Figure 4. Sp110 regulates genes involved in regulating apoptosis and intracellular survival of Mtb.

Figure 4

(a) RAW-Control and RAW-Sp110 cells were infected with H37Ra at a multiplicity of infection of 5:1 for 24 h and then expression levels of the genes indicated were determined by qPCR. (b) The RNA-seq levels of a selection of apoptosis regulators in uninfected or H37Ra-infected RAW-control and RAW-Sp110 cells. (c) The RNA-seq levels of selected genes associated with cell death regulation, cell cycle arrest, and cell proliferation regulation in uninfected or H37Ra-infected RAW-control and RAW-Sp110 cells. (d) Validation of DEGs by qPCR. (e) RNA-seq levels of the genes involved in regulating the intracellular growth of Mtb in uninfected or H37Ra-infected RAW-control and RAW-Sp110 cells. (f) Validation of the DEGs associated with intracellular growth of Mtb by qPCR. qPCR data are normalized to the uninfected RAW-Control and represent the mean ± SD of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001, compared with controls.