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. 2016 Feb 25;6:22041. doi: 10.1038/srep22041

Figure 5. Sp110 regulates miRNA expression in uninfected and Mtb-infected macrophages.

Figure 5

(a) The experimental scheme used for studying the miRNA expression profiles of RAW-Control and RAW-Sp110 cells in response to Mtb infection. Small RNA sequencing was performed on the uninfected and the H37Ra-infected RAW-Control and RAW-Sp110 cells at a multiplicity of infection of 5:1 for 24 h. Differentially expressed miRNAs were screened according to the groups indicated. DEmiR, differentially expressed miRNA. (b) Differences in the miRNA profiles of the H37Ra-infected RAW-Control/RAW-Sp110 cells and their uninfected counterparts were determined by small RNA sequencing. Selected DEmiRs are presented in a heat map. Green and red indicate low and high expression, respectively. (c) Venn diagrams showing the numbers of DEmiRs in uninfected RAW-Sp110 cells and in Mtb-infected RAW-Sp110 cells, with reference to basal expression levels in the uninfected RAW-Control cells. (d) Sp110 inhibits Mtb-induced miR-27b and miR-29a expression. (e) Sp110 enhances miR-146a and miR-155 expression. (f) Sp110 inhibits miR-125a, miR-21a and miR-99b expression. Expression of each miRNA in the uninfected and the H37Ra-infected RAW-Control and RAW-Sp110 cells was determined by qPCR. UI, uninfected cells; I, H37Ra infected cells. qPCR data represent three independent experiments. Error bars indicate mean ± SD (n = 3), *p < 0.05; **p < 0.01; ***p < 0.001, compared with controls.