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. 2016 Feb 25;6:21731. doi: 10.1038/srep21731

Figure 4. Apigenin regulated the expression of STAT3 target genes.

Figure 4

Cells were treated with various concentrations of apigenin for 24 h, and then (A) the enzymatic activities of MMP-2 and MMP-9 were determined by gelatinase zymography, (B) the mRNA expression level of MMP-2 was measured by RT-PCR, the protein levels of (C) MMP-2 and (D) VEGF were determined by immunoblotting, the relative protein level of MMP-2 was analyzed by Image J software and shown as mean ± S.D., **P < 0.01. (E) Twsit1 mRNA expression level and (F) Twist1 protein expression level were determined by RT-PCR and Western blotting, respectively. (G) A375 cells were transfected with a Twist1 promoter construct together with a PRL-CMV construct for 12 h, and then exposed to various concentrations of apigenin for another 12 h. The Twist1 transcriptional activity was measured by the dual-luciferase reporter assay. Data were shown as mean ± SD from three independent experiments, *P < 0.05 and **P < 0.01.