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. 2016 Feb 25;6:21731. doi: 10.1038/srep21731

Figure 5. Apigenin treatment partially reversed EMT in A375 cells.

Figure 5

(A) A375 cells were treated with indicated concentrations of apigenin for 24 h, and the mRNA expression levels of several EMT markers were determined by real-time PCR. Data were shown as mean ± SD from three independent experiments, *P < 0.05 and **P < 0.01. (B) After apigenin treatment for 24 h, the protein expression levels of EMT markers in A375 cells were determined by Western blotting. (C) A375 cells were transfected with a Twist1-specific siRNA (si-Twist1) or a control siRNA (si-Con) for 24 h, the mRNA expression levels of several EMT markers were determined by real-time PCR. Data were shown as mean ± SD from three independent experiments, **P < 0.01.