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. 2016 Feb 25;6:21731. doi: 10.1038/srep21731

Figure 7. Overexpression of STAT3C reversed apigenin-mediated inhibition on cell migration and invasion, and reduced the apigenin-mediated Twist 1 inhibition.

Figure 7

(A) A375 cells were transiently transfected with an empty vector or a STA3TC-expressing construct for 24 h, and then lysed for the Western blot analysis by using antibodies specific to p-STAT3, Flag, STAT3 and β-actin. (B) After transfection, cells were treated with apigenin for 24 h, and the effects of apigenin on Twist1 were determined by immunoblotting. The relative expression levels were analyzed by Image J software and shown as mean ± S.D., *P < 0.05, **P < 0.01. (C) Cell migratory and (D) invasive capacities were measured by the Transwell chamber assay. Representative photographs of migrated or invasive cells (upper) and quantification of these cells (bottom) were shown. Data were mean ± SD from three independent experiments, #P < 0.05 ##P < 0.01 and **P < 0.01.