Table 1. Thermodynamic parameters of porphyrin arrays formed from homo- or hetero-duplex systems ^a .

	ODN duplex	T m/°C [ΔT m]	ΔH/kJ mol–1 [ΔΔH]	–T 298ΔS/kJ mol–1 [Δ(–T 298ΔS)]	ΔG 298/kJ mol–1 [ΔΔG 298]
a	U1 5′-GTG ATA TGC	37.6	–279 ± 0.2	233	–46.4
	U2 3′-CAC TAT ACG	39.7 b	–285 ± 12 b	239 b	–45.9 b
b	R1 5′-GTG ARA TGC	32.5 [–5.1]	–261 ± 1 [+18]	220 [–13]	–40.7 [+5.7]
	U2 3′-CAC TAT ACG
c	U1 5′-GTG ATA TGC	33.0 [–4.6]	–281 ± 0.6 [–2]	239 [+6]	–41.9 [+4.5]
	R2 3′-CAC RAT ACG	34.5 b [–5.2]	–283 ± 6 b [+2]	241 [+2]	–42.1 b [+4.3]
d	U1 5′-GTG ATA TGC	31.4 [–6.2]	–255 ± 0.7 [+24]	215 [–18]	–40.3 [+6.1]
	R3 3′-CAC TAR ACG
e	U1 5′-GTG ATA TGC	27.7 [–9.9]	–254 ± 0.5 [+25]	217 [–16]	–36.6 [+9.8]
	R4 3′-CAC RAR ACG
f	F1 5′-GTG AFA TGC	36.7 [–0.9]	–356 ± 0.2 [–77]	308 [+75]	–47.7 [–1.3]
	U2 3′-CAC TAT ACG
g	U1 5′-GTG ATA TGC	35.7 [–1.9]	–257 ± 0.3 [+22]	214 [–19]	–42.6 [+3.8]
	F2 3′-CAC FAT ACG
h	U1 5′-GTG ATA TGC	35.4 [–2.2]	–333 ± 0.1 [–54]	287 [+54]	–45.9 [+0.5]
	F3 3′-CAC TAF ACG
i	U1 5′-GTG ATA TGC	29.9 [–7.7]	–203 ± 0.7 [+76]	165 [–68]	–37.5 [+8.9]
	F4 3′-CAC FAF ACG	28.9 b [–10.8]	–222 ± 7 b [+63]	186 b [–53]	–35.7 b [+10.7]
j	R1 5′-GTG ARA TGC	35.6 [–2.0]	–357 ± 0.8 [–78]	310 [+77]	–47.2 [–0.8]
	R2 3′-CAC RAT ACG
k	R1 5′-GTG ARA TGC	35.5 [–2.1]	–368 ± 0.2 [–89]	321 [+88]	–46.9 [–0.5]
	R3 3′-CAC TAR ACG
l	R1 5′-GTG ARA TGC	39.7 [+2.1]	–362 ± 0.2 [–83]	312 [+79]	–50.4 [–4.0]
	R4 3′-CAC RAR ACG
m	F1 5′-GTG AFA TGC	36.8 [–0.8]	–344 ± 0.2 [–65]	297 [+64]	–46.7 [–0.3]
	F2 3′-CAC FAT ACG
n	F1 5′-GTG AFA TGC	35.9 [–1.7]	–256 ± 0.3 [+23]	213 [–20]	–42.5 [+3.9]
	F3 3′-CAC TAF ACG
o	F1 5′-GTG AFA TGC	40.9 [+3.3]	–274 ± 0.2 [+5]	226 [–7]	–48.3 [–1.9]
	F4 3′-CAC FAF ACG
p	R1 5′-GTG ARA TGC	37.7 [+0.1]	–344 ± 0.2 [–65]	295 [+62]	–48.5 [–2.1]
	F2 3′-CAC FAT ACG
q	R1 5′-GTG ARA TGC	37.8 [+0.2]	–270 ± 0.2 [+9]	225 [–8]	–45.5 [+0.9]
	F3 3′-CAC TAF ACG	38.2 b [–1.5]	–348 ± 4 b [–63]	300b [+61]	–47.8 b [+1.4]
r	R1 5′-GTG ARA TGC	41.3 [+3.7]	–349 ± 0.1 [–70]	297 [+70]	–52.0 [–5.6]
	F4 3′-CAC FAF ACG
s	F1 5′-GTG AFA TGC	38.0 [+0.4]	–280 ± 0.5 [–1]	234 [+1]	–46.3 [+0.1]
	R2 3′-CAC RAT ACG
t	F1 5′-GTG AFA TGC	40.5 [+2.9]	–269 ± 0.4 [+10]	221 [–12]	–47.8 [–1.4]
	R3 3′-CAC TAR ACG
u	F1 5′-GTG AFA TGC	42.6 [+5.0]	–287 ± 0.4 [–8]	237 [–4]	–50.3 [–3.9]
	R4 3′-CAC RAR ACG

^aData obtained by thermal denaturing using UV monitoring.

^bData obtained by thermal denaturing using CD monitoring; ΔT _m is calculated relative to the base value obtained by the same method. The T _m values were obtained from the first derivative of the melting curves at 260 nm (0.1 °C min^–1, no hysteresis was observed; 2.5 μM DNA, 50 mM phosphate buffer, 100 mM NaCl, 1 mM Na₂EDTA, pH 7.0).