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. Author manuscript; available in PMC: 2016 Feb 25.
Published in final edited form as: Int J Parasitol. 2008 Nov 27;39(6):683–692. doi: 10.1016/j.ijpara.2008.11.002

Fig. 4.

Fig. 4

Fig. 4

Localisation of the PfRON6 protein in parasitised red blood cells (RBCs). (A) Localisation of PfRON6 in segmented schizonts. Confocal microscopy using anti-rPfRON6-A, -B and -C antisera. (B) Double labelling using anti-rPfRON6-B and anti-haemagglutinin (HA) in HA-tagged transgenic parasites. Confocal microscopy using anti-rPfRON6-B with anti-HA. Corresponding overlay images are shown. (C) Double labelling of PfRON6 with Pf34, PfRON4, RAMA or AMA1 in segmented schizonts. Confocal microscopy using anti-rPfRON6-B with anti-Pf34 (rhoptry neck marker), anti-PfRON4 (rhoptry neck marker), anti-RAMA (rhoptry bulb marker), or anti-AMA1 (microneme marker). Corresponding overlay images are shown. (D) Localisation of PfRON6 in free merozoites. Overlay images of free merozoites labelled with anti-rPfRON6-B, anti-RAMA and DAPI. (E) Electron micrograph of immunogold staining using affinity purified anti-rRON6-C antibodies. Arrows indicate PfRON6. (F) Localisation of PfRON6 during the asexual red blood cell (RBC) cycle. Immunofluorescence microscopy using anti-rPfRON6-B antisera and DAPI on parasites at different stages of the asexual RBC cycle. The corresponding phase contrast images are shown.