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. 2016 Feb 24;16:23. doi: 10.1186/s12896-016-0254-0

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© York et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — fGly conversion is affected by cell culture media and time in culture. A CHO-S clone (clone 101) stably expressing FGE + KDEL and CT-tagged antibody was cultured in PF-CHO™ or FortiCHO™, fed with 10 % Efficient Feed C on Days 3, 5, and 7, and supplemented with 3 g/L glucose when levels dropped below 3 g/L. Samples of media were taken at the indicated days (harvest was at day 11 and day 14 for FortiCHO™ and PF-CHO™ cultures, respectively). Titers (indicated by the numbers atop bars, mg/L) were assessed by ELISA, and conversion was determined by mass spectrometry. Representative data are shown (n =2 experiments)