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. 2016 Feb 24;16:23. doi: 10.1186/s12896-016-0254-0

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© York et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — fGly conversion proceeds efficiently in cells cultured in the presence of 5–50 μM copper(II) sulfate. CHO-S clone 101 stably expressing FGE + KDEL and CT-tagged antibody was cultured in FortiCHO™ +/- the indicated concentrations of copper(II) sulfate added on day 0 (a), or with 50 μM copper(II) sulfate added on days 3 or 5 (b). Cultures were fed with 10 % Efficient Feed C on Days 3, 5, and 7. Media was harvested at day 10 (a) or day 12 (b); titers (indicated by the numbers atop bars, mg/L) were assessed by ELISA, and conversion was determined by mass spectrometry. n = 7 experiments for 0 and 50 μM copper(II) sulfate added on day 0; other time points and copper(II) sulfate concentrations were tested once