Fig. 7.

PA-induced lipotoxicity and OA protection against PA-induced lipotoxicity in perilipin 1 or 2 suppressed insulin-producing RINm5F and INS-1E cells. Perilipin 1 or 2 suppressed RINm5F cells were incubated with increasing concentrations of the saturated NEFA C16:0 (PA) (a) or co-incubated with 200 μM PA and increasing concentrations of the monounsaturated NEFA C18:1 (OA) (c). Perilipin 1 or 2 suppressed INS-1E cells were incubated with increasing concentrations of the saturated NEFA C16:0 (PA) (b) or co-incubated with 500 μM PA and increasing concentrations of the monounsaturated NEFA C18:1 (OA) (d). The cell viability was measured by MTT assay after 24 h. Data are means ± SEM of n = 4 to 6. Significance was tested against control cells (ANOVA/Tukey Multiple Comparison Test)