Fig. 8.

RGD of gB is required for attachment of cells. a KSHV gBΔTM protein-induced cell adhesion. Maxisorp plates were coated with different concentrations of purified gBΔTM, gBΔTMΔR, gBΔTMΔD, or BSA proteins overnight at 4 °C, washed, and cell adhesion assays were carried out. Each point on the plot represents the average from five experiments. b Antibodies to RGD inhibit the HeLa cell adhesion to gBΔTM protein. Maxisorp plates were coated with 4 μg of purified gBΔTM protein/ml overnight at 4 °C, washed, and incubated for 1 h at 4 °C with different antibodies prior to seeding the plate with HeLa cells and performing the cell adhesion assay. Data are presented as percentage of inhibition of cell adhesion obtained when gBΔTM was preincubated with DMEM only. Each point on the plot represents the average from five experiments. c Anti-RGD antibodies block the ability of gB expressed on the surfaces of HeLa cells to promote cell adhesion. HeLa cells expressing full length gB were incubated with various antibodies for 1 h at +4 °C prior to performing cell adhesion assay in a 24-well plate. Data are presented as percentage cell adhesion observed when cells expressing gB were preincubated with DMEM only. d gBΔR does not promote cell adhesion. HeLa cells expressing gB, gBΔR, and gBΔD were tested in adhesion assay. Data are presented as percentage cell attachment observed when cells expressing gB were tested. Each point on the plot represents the average from five experiments. Column with asterisk mark denotes the value to be statistically significant (p < 0.05) by LSD