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. Author manuscript; available in PMC: 2016 Feb 25.
Published in final edited form as: J Immunol. 2014 Jun 18;193(2):645–654. doi: 10.4049/jimmunol.1301836

Figure 7. STAT6 is crucial for IL-31 expression in Th2 cells.

Figure 7

Human in vitro-polarized Th2 cells were transfected with siRNA targeting STAT6 (siSTAT6) or control oligonucleotide (siControl). Four days post-transfection, cells were replated in fresh medium and re-stimulated under Th2 conditions (α-CD3/α-CD28 + IL-4 + α-IL-12/α-IL-23) for 24 hours. A. Western blot analyses showing reduced levels of phospho-STAT6 (p-STAT6), STAT6 and GATA-3 in STAT6-silenced Th2 cells. B. Real-time q-RT-PCR analyses of mRNA expression of Stat6, Gata3, Il4, Il5, Il13 and Il31. Data represent 6 experiments carried out in duplicates using cells from 6 individual donors.