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. Author manuscript; available in PMC: 2016 Feb 25.
Published in final edited form as: ACS Synth Biol. 2015 Oct 30;5(1):53–64. doi: 10.1021/acssynbio.5b00119

FIGURE 1. Binding affinities of lit and dark states highlight difference in photoswitch dynamic range.

FIGURE 1

Fluorescence polarization binding plots for A) LOVpep constructs and ePDZb (left) iLID nano and micro (middle) and CRY2 and CIB1N (right). B) Fluorescence polarization of each complex was measured under blue light (blue) or darkness (black) to determine binding affinity. C) Affinity values from binding data plotted on a Dynagram highlight the dynamic range of each tool.