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. Author manuscript; available in PMC: 2016 Feb 25.
Published in final edited form as: ACS Synth Biol. 2015 Oct 30;5(1):53–64. doi: 10.1021/acssynbio.5b00119

FIGURE 5. Targeted mitochondrial localization identifies differences in dark state binding dynamic range and kinetics.

FIGURE 5

A) Representative images of the data analyzed in B. Cells transfected with each mitochondrial bound switch pair were visualized and activated by confocal microscopy. Venus labeled constructs are bound to the plasma membrane while tgRFPt labeled constructs are cytoplasmic. The entire field of view is activated. The activation and post activation images represent the final image of the specified time frame. (Bar = 50 μm) B) A ratio of mitochondrial to cytoplasmic tgRFPt fluorescence intensity throughout the experiments shown in A.