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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Pain. 2015 Jun;156(6):1153–1160. doi: 10.1097/j.pain.0000000000000171

Figure 3.

Figure 3

Nest consolidation under control conditions and after treatment with noxious stimuli. Panel A shows Number of Zones Cleared from 1 to 100 min after no treatment (N=8) or after control treatments for noxious stimuli (i.p. saline administered 5 min before nesting, N=12; brief isolflurane anesthesia 24 hr before nesting, N=9). Treatment was a between-subjects factor, and time was a within-subjects factor. Filled points indicate significantly different from “No Treatment” at a given time point (p<0.05 for this and all other post hoc comparisons after a significant 1- or 2-way ANOVA). Panel B shows effects of i.p. lactic acid concentration (0–0.32%, N=6). Acid concentration was a within-subjects factor, such that all mice received all acid concentrations. Filled points indicate significantly different from saline treatment (Sal). Panel C shows the time course of effects produced by i.p. saline or 0.32% lactic acid (LA). Both treatment and time were between-subjects factors, and N=6 for conditions. Filled points indicate significantly different from saline at a given time point. Panel D shows the time course of effects produced by anesthesia alone or anesthesia in combination with bilateral intraplantar saline, unilateral CFA (with saline injected in the opposite paw), or bilateral CFA. Treatment was a between-subjects factor and time was a within-subjects factor, and N=9 for all conditions. Filled points indicate significantly different from the anesthesia only control at a given time point.