Table 3. The effects of butanol on imbibitional chilling injury in soybean seeds.

n-, s-, and t-butanol were applied to R5 seeds and their effects on the germination rate were assessed. Each of the three butanol isoforms was applied to the seeds immersed in water (I-W) at 4 °C for 0, 3, and 24 h and then they were transferred to moistened paper at 25 °C for one day of post-germination (P-G). Data are shown as mean ± SE (n = 4 replicates of 40 seeds each). Values in the same row with an asterisk are significantly different from that of control (Water) (P < 0.05).

Reagent	Concentration (%)	Germination (%)
		C	3 h (I-W)	24 h (I-W)	1 d (P-G)
Water	0	0	0	0	0
n-butanol	0.01	10.8 ± 2.2*	10.4 ± 2.0*	0	0
	0.05	15.1 ± 3.9*	12.3± 3.1*	0	0
	0.08	13.1 ± 2.1*	10.6± 3.0*	0	0
s-butanol	0.01	0	0	0	0
	0.05	0	0	0	0
	0.08	0	0	0	0
t-butanol	0.01	0	0	0	0
	0.05	0	0	0	0
	0.08	0	0	0	0