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. Author manuscript; available in PMC: 2016 Feb 25.
Published in final edited form as: Plant Cell Environ. 2015 Jan 25;38(7):1391–1403. doi: 10.1111/pce.12494

Table 5. The effects of exogenous NAE and butanol on germination of cucumber and pea seeds during imbibitional chilling.

NAE in DMSO solvent and n- and t-butanol were applied to the seeds immersed in water at 4 °C for 24 h, which were then transferred to moistened paper at 25 °C and scored for their relative germination rate at seven days. Data are shown as mean ± SE (n = 4 replicates of 40 seeds each). Values in the same row with an asterisk are significantly different from that of control (Water) (P < 0.05).

Reagent Concentration Germination (%)
Cucumber Pea
Water 0 37.78 ± 4.69 19.44 ± 2.81

DMSO 5 µmol 35.16 ± 6.73 18.91 ± 3.39
10 µmol 34.72 ± 5.91 20.64 ± 4.24
25 µmol 37.26 ± 6.02 20.98 ± 3.51

NAE 5 µmol 57.55 ± 8.42* 49.30 ± 7.36*
10 µmol 58.72 ±9.38* 43.39 ± 8.75*
25 µmol 60.19 ±10.45* 40.29 ± 9.42*

n-butanol 0.01% 49.57 ± 5.42* 32.70 ± 4.64*
0.05% 47.48 ± 7.41 30.74 ± 5.38*
0.08% 48.82 ± 8.33 32.48 ± 5.36*

t-butanol 0.01% 35.92 ± 6.47 20.05 ± 3.18
0.05% 34.07 ± 7.42 21.95 ± 3.38
0.08% 33.39 ± 4.19 22.35 ± 4.05