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. Author manuscript; available in PMC: 2016 Jul 3.
Published in final edited form as: Science. 2015 Jul 3;349(6243):91–95. doi: 10.1126/science.aab0515

Figure 4.

Figure 4

The BC-box proteins contribute to CRL2 recognition of truncated selenoproteins. (A) GPS assay for cells carrying UGA-terminated (Δ) or full-length (FL) selenoproteins infected with viruses expressing various BC-box proteins. (B) GPS assay for cells expressing Δ or FL selenoproteins treated with shRNAs against BC-box proteins. FL and Δ selenoproteins were presented using different x-axis scales. (C) GST pull-down using lysates expressing HA-tagged BC-box proteins and GST or GST-tagged Δ or FL selenoproteins. (D) GPS assay for cells expressing various forms of SELV or SELK infected with viruses expressing VHL, APPBP2, or KLHDC2. The truncation sites relative to Sec are labeled above. (E) GST pull-down using lysates expressing HA-tagged KLHDC2 and GST or various forms of GST-tagged SELK. (F) GST pull-down using lysates expressing GFP-tagged GAPDH-CTT fusion and GST or GST-tagged BC-box proteins. (G) The protein stability of truncated selenoproteins terminated with various CTTs. (H) The stability of UGA-terminated selenoproteins in various cells.