Table 1. Primers and probes used for Neisseria meningitidis serogrouping of isolates from samples from a national register for molecular surveillance of invasive bacterial disease, Italy, 2007–2014.

Target	Gene	Forward primer	Reverse primer	Probe
N. meningitidis	ctrA	gctgcggtaggtggttcaa	ttgtcgcggatttgcaacta	FAM_cattgccacgtgtcagctgcacat_TAMRA
Serogroup A	sacB	cccccagcatggctagattt	agggcactttgtggcataattt	FAM_accctaaaattcaatgggtatatcacga_TAMRA
Serogroup B	siaD B	ttggacttggttaagctgacctaa	gttgacaacatctccattttatcttacc	FAM_ttagatatgacaaataaattgttacgtggg_TAMRA
Serogroup C	siaD C	agggaaccgcaacctatgc	cacaaaacgttgctcaaattttg	FAM_ccactcttagaatcatttacatacaaaccc_TAMRA
Serogroup W/Y	siaD W/Y	gctgataaattgttcttatggtctgaa	cggcaccagaaccaatctct	FAM_ttggaatcatgagcttttaccaaatccaaca_TAMRA
Serogroup W*	siaD	cagaagtgagggatttccata	cacaaccattttcattatagttactgt
Serogroup Y*	siaD	ctcaaagcgaaggctttggtta	ctgaagcgttttcattataattgctaa

*Identified by using endpoint PCR.