Figure 2.
Partitioning of raftophillic integral membrane proteins in giant unilamellar vesicles. GUVs were formed from liposomes containing a 2:2:1 molar ratio of POPC:PSM:cholesterol along with 0.1 mol % of the fluorescent tracer lipid rhodamine-DOPE (magenta, Lα phase) and various membrane proteins at a bulk molar lipid: protein ratio of 400:1. (A) A representative image of GUVs containing AF488-labeled caveolin-3 (yellow) are shown along with a 10 μm scale bar for reference. (B) A representative image of GUVs containing AF488-labeled C99 (yellow) are shown along with a 10 μm scale bar for reference. (C) A representative image of GUVs containing AF488-labeled PMP22 (yellow) are shown along with a 10 μm scale bar for reference.