TABLE 1.

Summary of the analytical parameters selected for the detection of Apos

Apo	Peptide Sequence	Fragment	Cone/Collision (V)	MRM Transition (m/z)	Retention Time (min)
ApoA-I
M0	ATEHLSTLSEK	y6+	30/25 V	608.3 → 664.3	1.6 ± 0.1
M3	ATEH L ST L SEK			609.8 → 664.3 + 667.3
IS	ATEHLSTLSEK			612.3 → 672.3
ApoA-II
M0	SPELQAEAK	y6+	30/30 V	486.8 → 659.3	1.4 ± 0.1
M3	SPE L QAEAK			488.3 → 662.3
IS	SPELQAEAK			490.3 → 667.3
ApoB100
M0	NLQNNAEWVYQGAIR	y6+	50/30 V	888.5 → 707.4	2.7 ± 0.1
M3	N L QNNAEWVYQGAIR			890.0 → 707.4
IS	NLQNNAEWVYQGAIR			893.5 → 717.4
ApoC-II
M0	TAAQNLYEK	y4+	80/30 V	1,037.9 → 552.3	1.4 ± 0.1
M3	TAAQN L YEK			1,040.9 → 555.3
IS	TAAQNLYEK			1,045.9 → 560.3
ApoC-III
M0	DALSSVQESQVAQQAR	y8+	40/35 V	858.9 → 887.5	2.0 ± 0.1
M3	DA L SSVQESQVAQQAR			860.4 → 887.5
IS	DALSSVQESQVAQQAR			863.4 → 897.5
ApoE
M0	LGPLVEQGR	y5+	25/30 V	484.8 → 588.3	2.1 ± 0.1
M3	L GP L VEQGR			486.3 → 588.3
IS	LGPLVEQGR			489.3 → 598.3

Underlined L indicates the putative incorporation site(s) of ²H₃-leucine. Bold indicates the labeled amino acid [¹³C₆, ¹⁵N₂]K or [¹³C₆, ¹⁵N₄]R. IS, internal standard.