Effects of CU-1, CU-2, CU-3, and CU-4 on DGKα activity. Purified DGKα (1.25 μg) was incubated with various concentrations of CU-1 (A), CU-2 (B), CU-3 (C), or CU-4 (D) as indicated for 5 min. Values are the average of duplicate determinations, which were within 5% of the mean. Similar results were obtained in two repeated experiments (experiments 1 and 2).