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Effects of CU-3 on the activities of the DGKα truncation mutants. A: Schematic presentation of DGKα truncation mutants. RVH, recoverin homology. B: The 12,000 g supernatant (5 μg) of the extracts from COS-7 cells expressing wild-type DGKα, DGKα-Δ1–196, or DGKα-Δ1–332 was incubated for 5 min in the presence (1 μM) or absence (DMSO alone) of CU-3. The activities of wild-type DGKα, DGKα-Δ1–196, and DGKα-Δ1–332 in the absence of CU-3 are defined as 100%.
