TABLE 1.
MS/MS instrument parameters
| Capillary voltage | 3.50 KV |
| Cone voltage | 20 V |
| Source temperature | 120°C |
| Desolvation temperature | 450°C |
| Cone gas | 50 l/h |
| Desolvation gas | 950 l/h |
| Collision gas | 0.20 ml/min |
| Collision voltage | 20 V |
| Type | Multiple reaction monitoring |
| Ion mode | ES+ (electrospray positive) |
| Dwell time | 0.250 s |
| Interchannel delay | 0.005 s |
| Interscan delay | 0.005 s |
| Transitions | |
| GlcChol, RT (min) | 1.43 |
| 13C6-GlcChol, RT (min) | 1.43 |
| Fit weight, RT (min) | None |
| Smooth method, RT (min) | Mean |
| Smooth width, RT (min) | 2 |
RT, retention time.