Fig. 2.

Treatment with the β3-adrenergic receptor agonist, CL, and cold exposure activate thermogenesis in BAT, induce browning in WAT, and alter endocannabinoid receptor expression in both adipose tissues. In order to activate BAT and/or browning of WAT, C57BL6/J wild-type mice housed at ambient temperature (22–24°C) received a single injection of CL (acute CL), 7 days of CL treatment (chronic CL), or the combination of both (chronic+acute CL), as described in the Methods. To stimulate BAT and/or WAT browning by cold, C57BL6/J wild-type mice were housed for 1 day (1d cold) or 7 days (7d cold) at 6°C. Expression of brown adipocyte activation marker genes relative to mock-injected controls was determined in BAT (A–D) and in subcutaneous WAT (E–H). I–L: Relative expression of endocannabinoid receptors Cnr1 and Cnr2 in BAT and WAT. Values are mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001, Student’s t-test.