Fig. 7.

Acute β3-adrenergic treatment of primary brown adipocytes significantly increases Cnr1 and 2-AG synthesis gene expression. Adipocytes were treated with saline or 1 μM CL for 4 h. A: Concentrations of unesterified fatty acids (FFA) were determined in cell supernatants. B–J. Relative mRNA expression of Ucp1, Cnr1, and endocannabinoid synthetic and degrading enzymes. Results are presented as means ± SEM from triplicates of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, Student’s t-test.