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. Author manuscript; available in PMC: 2016 Feb 25.
Published in final edited form as: Nat Genet. 2015 May 11;47(6):615–624. doi: 10.1038/ng.3293

Figure 5.

Figure 5

Pten loss accelerates osteosarcoma development in mice and PTEN loss enhances the anchorage-independent growth of immortalized human osteoblast cells. (a) Osteosarcoma-free survival curve depicting time to osteosarcoma development and survival endpoints for Trp53-C, Trp53-Pten and Pten mice. ***P < 0.0001, log-rank test. (b) Representative gross necropsy image of a primary osteosarcoma demonstrating typical gross morphology (left) and a section stained with hematoxylin and eosin (right) demonstrating a histological appearance consistent with osteosarcoma. Scale bar, 200 μm. (c) Diagram of the experimental procedure used to knock out PTEN with TALENs in immortalized human osteoblast cells. (d) Average number of colonies formed in soft agar by immortalized osteoblast cells treated with PTEN or HPRT1 TALENs. Data are the means ± s.e.m. of five independent experiments; ***P < 0.0001, Student's t test. Error bars, s.d.