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. Author manuscript; available in PMC: 2016 Oct 7.
Published in final edited form as: FEBS Lett. 2015 Sep 7;589(20 0 0):3212–3219. doi: 10.1016/j.febslet.2015.08.041

Figure 4. Dexras1 phosphorylation attenuates NO-mediated iron uptake via Dexras1.

Figure 4

HEK293T cells were transfected with Dexras1-myc and pre-treated with forskolin (1 and 10 uM) for 1 hour and then further treated with GSNO (100 uM) for 1 hour. (A) Cells were subjected to biotin switch assay or (B) iron uptake assay. Iron uptake experiments were repeated five times, each sample in at least triplicate. S-nitrosylation assay was performed three times.