Figure 1. CD24⁺CD44⁻ cells are highly resistant to irradiation and chemotherapy.

A. Chemotaxis of CD24⁺CD44⁻, CD24⁻CD44⁺, and CD24⁻CD44⁻ cells sorted from the A2780 cell line across Transwell membranes in response to conditioned media (CM) harvested from BM or liver of irradiated C57BL6 mice. B. Chemotaxis of CD24⁺CD44⁻, CD24⁻CD44⁺, and CD24⁻CD44⁻ cells sorted from the A2780 cell line exposed to irradiation (1000 cGy) and cisplatin (10 μm for 48 hours) and allowed to migrate across Transwell membranes in response to CM from irradiated BM. The chemotaxis assay was done at least thrice in duplicate, with similar results.