Figure 3. Expansion of single cells sorted from the R2, R4, and R5 flow cytometry regions of the parental A2780 cell line stained with anti-CD24 and anti-CD44 antibodies.

Fluorescence-activated cell sorting analysis of cultures derived from singly sorted CD24⁺CD44⁻, CD24⁻CD44⁺, and CD24⁻CD44⁻ cell phenotypes from the A2780 cell line demonstrate that singly sorted cells, regardless of their phenotype, reestablish the phenotypes of the parental cell line. A representative analysis out of three experiments performed in triplicate is shown.