Table 2. Molecular identification of the strains used in this study, including the percentual of identity, accession number and the name of the identified species on the Barcode of Life Database (based on rbcL marker sequence) and GenBank (based on nuITS2 marker sequence).
| Strain | ITS1 (GenBank) | ITS2 (GenBank) | rbcL (BOLD) | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Closest match species | Identity | GenBank access | Closest match species | Identity | Number of CBCs / hCBCs | GenBank access | Closest match species | Identity | GenBank access | |
| LBA#1 | Desmodesmus armatus | 95% | KP281288.1 | Desmodesmus bicellularis | 91% | 1 / 7 | AB917134.1 | Scenedesmus quadricauda | 90% | AB084332.1 |
| LBA#2 | Desmodesmus sp. MAT-2008c | 100% | EU502836.1 | Desmodesmus sp. MAT-2008c | 100% | 0 / 0 | EU502836.1 | Acutodesmus obliquus | 93% | DQ396875.1 |
| LBA#3 | Desmodesmus sp. MAT-2008c | 100% | EU502836.1 | Desmodesmus sp. MAT-2008c | 100% | 0 / 0 | EU502836.1 | Acutodesmus obliquus | 90% | DQ396875.1 |
| LBA#4 | Chlamydopodium starrii | 70% | AB983644.1 | Chlorococcum oleofaciens | 91% | 1 / 2 | AB983633.1 | Chlorococcum ellipsoideum | 91% | EF113431.1 |
| LBA#5 | Desmodesmus sp. Tow 10/11 T-12W | 79% | DQ417556.1 | Desmodesmus regularis | 84% | 4 / 2 | AM228924.1 | Desmodesmus santosii | 93% | GU192417.1 |
| LBA#6 | Chlamydopodium starrii | 70% | AB983644.1 | Chlorococcum oleofaciens | 94% | - | AB983633.1 | Chlorococcum ellipsoideum | 91% | EF113431.1 |
| LBA#7 | Desmodesmus sp. Tow 10/11 T-12W | 79% | DQ417556.1 | Desmodesmus regularis | 84% | 4 / 2 | AM228924.1 | Desmodesmus santosii | 93% | GU192417.1 |
| LBA#8 | Chlamydomonas sp. KU107 | 94% | KM061447.1 | Chlamydomonas sp. KU107 | 87% | 0 / 1 | KM061447.1 | Chlamydomonas oblonga | 95% | EF113424.1 |
| LBA#9 | Chlamydopodium starrii | 90% | AB983644.1 | Chlamydopodium starrii | 93% | 0 / 1 | AB983644.1 | Chlorococcum ellipsoideum | 92% | KC810301.1 |
| LBA#10 | Chlamydopodium starrii | 90% | AB983644.1 | Chlamydopodium starrii | 93% | 0 / 1 | AB983644.1 | Chlorococcum ellipsoideum | 92% | KC810301.1 |
| LBA#11 | Chlamydopodium starrii | 90% | AB983644.1 | Chlamydopodium starrii | 93% | 0 / 1 | AB983644.1 | Chlorococcum ellipsoideum | 92% | KC810301.1 |
| LBA#12 | Chlamydopodium starrii | 90% | AB983644.1 | Chlamydopodium starrii | 93% | 0 / 1 | AB983644.1 | - | - | - |
| LBA#13 | Coelastrella sp. shy-188 | 96% | KP702302.1 | Scenedesmus rubescens | 95% | 0 / 2 | JX513884.1 | Scenedesmus quadricauda | 90% | AB084332.1 |
| LBA#14 | Chlamydopodium starrii | 90% | AB983644.1 | Chlamydopodium starrii | 93% | 0 / 1 | AB983644.1 | Chlorococcum ellipsoideum | 92% | KC810301.1 |
| LBA#15 | Chlamydopodium starrii | 90% | AB983644.1 | Chlamydopodium starrii | 93% | 0 / 1 | AB983644.1 | Chlorococcum ellipsoideum | 92% | KC810301.1 |
| LBA#16 | - | - | - | - | - | - | - | Ecballocystopsis dichotomus | 90% | JX018187.1 |
| LBA#17 | Chlamydopodium starrii | 90% | AB983644.1 | Chlamydopodium starrii | 93% | 0 / 1 | AB983644.1 | Chlorococcum ellipsoideum | 92% | KC810301.1 |
| LBA#18 | Chlamydopodium starrii | 90% | AB983644.1 | Chlamydopodium starrii | 93% | 0 / 1 | AB983644.1 | Chlorococcum ellipsoideum | 92% | KC810301.1 |
| LBA#19 | - | - | - | - | - | - | - | Ecballocystopsis dichotomus | 90% | JX018187.1 |
| LBA#20 | Coelastrum astroideum | 76% | GQ375093.1 | Scenedesmus arcuatus | 81% | 0 / 6 | AY170855.1 | Hariotina reticulata | 93% | JQ394815.1 |
| LBA#21 | Coelastrella sp. shy-188 | 96% | KP702302.1 | Scenedesmus rubescens | 95% | 0 / 2 | JX513884.1 | Desmodesmus costato-granulatus | 94% | GU192427.1 |
| LBA#22 | Desmodesmus ultrasquamatus | 100% | GU192392.1 | Desmodesmus ultrasquamatus | 99% | 0 / 0 | GU192392.1 | Desmodesmus costato-granulatus | 93% | GU192427.1 |
| LBA#23 | Desmodesmus ultrasquamatus | 100% | GU192392.1 | Desmodesmus ultrasquamatus | 99% | 0 / 0 | GU192392.1 | Desmodesmus costato-granulatus | 94% | GU192427.1 |
| LBA#24 | Desmodesmus ultrasquamatus | 94% | GU192392.1 | Desmodesmus ultrasquamatus | 94% | 0 / 3 | AM228926.1 | Desmodesmus costato-granulatus | 94% | GU192427.1 |
| LBA#25 | Desmodesmus ultrasquamatus | 94% | GU192392.1 | Desmodesmus ultrasquamatus | 94% | 0 / 3 | AM228926.1 | Desmodesmus costato-granulatus | 94% | GU192427.1 |
| LBA#26 | Desmodesmus sp. MAT-2008c | 100% | EU502836.1 | Desmodesmus sp. MAT-2008c | 100% | 0 / 0 | EU502836.1 | Acutodesmus obliquus | 92% | DQ396875.1 |
| LBA#27 | Chlorella sorokiniana | 100% | KM061456.1 | Chlorella sorokiniana | 100% | 0 / 0 | KJ676113.1 | Chlorella sorokiniana | 99% | HM101339.1 |
| LBA#28 | - | - | - | - | - | - | - | Selenastrum sp. KMMCC 1456 | 94% | JQ315488.1 |
| LBA#29 | Chlorella sp. MAT-2008a | 92% | EU502833.1 | Chlorella sp. MAT-2008a | 91% | 0 / 2 | EU502833.1 | Chlorella sp. IFRPD 1018 | 93% | AB260911.1 |
| LBA#30 | Desmodesmus sp. MAT-2008c | 100% | EU502836.1 | Desmodesmus sp. MAT-2008c | 100% | 0 / 0 | EU502836.1 | Acutodesmus obliquus | 93% | DQ396875.1 |
| LBA#31 | Chlorella sp. MAT-2008a | 92% | EU502833.1 | Chlorella sp. MAT-2008ª | 91% | 0 / 2 | EU502833.1 | Chlorella sp. IFRPD 1018 | 93% | AB260911.1 |
| LBA#32 | Micractinium sp. CCAP 211/92 | 99% | FM205863.1 | Micractinium sp. CCAP 211/92 | 100% | 0 / 0 | FM205863.1 | Chlorella pyrenoidosa | 99% | FM205863.1 |
| LBA#33 | Micractinium sp. CCAP 211/92 | 99% | FM205863.1 | Micractinium sp. CCAP 211/92 | 100% | 0 / 0 | FM205863.1 | Chlorella pyrenoidosa | 99% | FM205863.1 |
| LBA#34 | Micractinium sp. CCAP 211/92 | 99% | FM205863.1 | Micractinium sp. CCAP 211/92 | 100% | 0 / 0 | FM205863.1 | Chlorella pyrenoidosa | 99% | FM205863.1 |
| LBA#35 | Desmodesmus sp. GM4a | 100% | AB917128.1 | Desmodesmus sp. GM4a | 99% | 0 / 1 | AB917128.1 | Desmodesmus baconii | 93% | KC315289.1 |
| LBA#36 | Desmodesmus sp. MAT-2008c | 100% | EU502836.1 | Desmodesmus sp. MAT-2008c | 100% | 0 / 0 | EU502836.1 | Acutodesmus obliquus | 93% | DQ396875.1 |
| LBA#37 | Chlamydomonas sp. YB3-2 | 90% | JN862852.1 | Chlamydomonas applanata | 92% | 1 / 2 | FR865616.1 | Ascochloris multinucleata | 94% | EF113411.1 |
| LBA#38 | Chlamydomonas sp. YB3-2 | 90% | JN862852.1 | Chlamydomonas applanata | 92% | 1 / 2 | FR865616.1 | Ascochloris multinucleata | 94% | EF113411.1 |
| LBA#39 | Chlorella sorokiniana KU207 | 100% | KM061456.1 | Chlorella sorokiniana | 100% | 0 / 0 | KJ676113.1 | Chlorella sorokiniana | 99% | HM101339.1 |
| LBA#40 | Chlamydomonas zebra | 79% | AF033294.1 | Chlamydomonas sp. XJU-36 | 95% | 2 / 0 | FJ572059.1 | Chlamydomonas orbicularis | 96% | AB511849.1 |
| LBA#41 | Chlamydomonas sp. KU107 | 94% | KM061447.1 | Chlamydomonas sp. KU107 | 87% | 0 / 3 | KM061447.1 | Chlamydomonas oblonga | 95% | EF113424.1 |
| LBA#42 | Micractinium sp. CCAP 211/92 | 99% | FM205863.1 | Micractinium sp. CCAP 211/92 | 100% | 0 / 0 | FM205863.1 | Chlorella pyrenoidosa | 99% | FM205863.1 |
| LBA#43 | Micractinium sp. CCAP 211/92 | 99% | FM205863.1 | Micractinium sp. CCAP 211/92 | 100% | 0 / 0 | FM205863.1 | Chlorella pyrenoidosa | 99% | FM205863.1 |
| LBA#44 | Micractinium sp. CCAP 211/92 | 99% | FM205863.1 | Micractinium sp. CCAP 211/92 | 100% | 0 / 0 | FM205863.1 | Chlorella pyrenoidosa | 99% | FM205863.1 |
| LBA#45 | Chlorococcum oleofaciens | 82% | AB983633.1 | Spongiochloris spongiosa | 86% | - | U34776.1 | Protosiphon botryoides | 92% | EF113465.1 |
| LBA#46 | Uronema sp. AF-2012 | 98% | JX092263.1 | Uronema trentonense | 100% | 0 / 0 | HF920659.1 | - | - | - |
| LBA#47 | Tetracystis tetraspora | 95% | KM020024.1 | Dunaliella sp. SPMO 300–4 | 85% | 2 / 0 | DQ377118.1 | Nautococcus solutus | 91% | AB360758.1 |
| LBA#48 | - | - | - | - | - | - | - | Gungnir sp. NIES-1851 | 93% | AB603749.1 |
| LBA#49 | Lobochlamys segnis | 83% | FR865604.1 | Chlamydomonas sp. CCAP 11/150 | 90% | 0 / 1 | FR865545.1 | Asterococcus korschikoffii | 90% | AB175944.1 |
| LBA#50 | Chlorella sp. KMMCC 1468 | 99% | JQ315774.1 | Chlorella sorokiniana | 96% | 0 / 0 | LK021940.1 | Chlorella sp. IFRPD 1014 | 99% | AB260910.1 |
| LBA#51 | Chlorococcum oleofaciens | 74% | AB983630.1 | Chlorococcum sp. CCAP 11/52 | 84% | 2 / 1 | FR865591.1 | Chlamydopodium vacuolatum | 95% | EF113426.1 |
The compensatory and hemi-compensatory base changes (CBCs/hemi-CBCs) between the indicated sequence and its closest match in the ITS2 Database are shown. An hyphen (-) is indicated for samples that could not be amplified and/or sequenced, and for the nuITS2 sequences for which secondary structure predictions and CBCs/Hemi-CBCs analysis were not possible.