Figure 2. Absence of platelet-neutrophil interactions after hepatic I/R in mice. Systemic triple staining and intravital imaging of platelets (red), endothelium (blue), and neutrophils (green) was performed as described in sections 2.2 and 2.3, and [B23]. Panels A and B show close-ups of I/R-induced platelet plugs and sinusoidal neutrophil adhesion, respectively. In contrast to recent venous thrombosis literature [B26], neutrophils did not mediate the formation of platelet aggregates after I/R, as evidenced by the lack of neutrophil-platelet co-location (C, D). Scale bar = 30 μm.