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GlioSeq next-generation sequencing (NGS) panel design. It includes 30 genes (>1360 CNS tumor- related hot spots) analyzed for point mutations and small insertions and deletions, 24 genes (in bold) for copy number changes, 16 subtypes of BRAF and FGFR3 gene fusions, and EGFRvIII structural alterations. In addition, it tests for the expression of 3 housekeeping genes (GUSB, HPRT1, PGK) for evaluation of RNA integrity.
