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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: J Investig Med. 2015 Aug;63(6):806–810. doi: 10.1097/JIM.0000000000000213

Figure 5. Hemoglobin depleted RBC lysates-induced IL-8 mRNA expression is regulated by IL-33 receptor expression.

Figure 5

RBC lysates were filtered through a 50 kDa cutoff Amicon Ultra column to remove hemoglobin. Beas2B cells were transfected with sST2 or ST2L plasmids for 48 h prior to treatment with the hemoglobin depleted filtrates from hemolyzed RBCs for additional 3 h and total RNA was extracted. IL-8 mRNA levels were determined by real-time PCR.