Table 1.
Designed primers for β‐ CA genomic sequences from T . spiralis, T . pseudospiralis and T . nativa.
| No. | Designed primers | Species name | Product length (bp) |
|---|---|---|---|
| 1 |
Forward: 5′‐AGAGACTGCCCGTTCACAAG‐3′ Reverse: 3′‐CTGGGAGAGTTTGTCAGCGT‐5′ |
T. spiralis | 882 |
| 2 |
Forward: 5′‐TTTGAGCGCACTAGCATCCA‐3′ Reverse: 3′‐TCCATTCTGCATCACGCTGT‐5′ |
T. spiralis | 191 |
| 3 |
Forward: 5′‐AGACTGCCCGTTCACAAGTT‐3′ Reverse: 3′‐TGGATGCTAGTGCGCTCAAA‐5′ |
T. spiralis | 530 |
| 4 |
Forward: 5′‐ATTAATAAGTGAAAAGCACA‐3′ Reverse: 3′‐GTTTTAGAACTGGACACTGT‐5′ |
T. spiralis | 83 |
| 5 |
Forward: 5′‐CTCACCCATCACCCCGGCTT‐3′ Reverse: 3′‐TCCATTCTGCATCACGCTGT‐5′ |
T. pseudospiralis | 251 |
| 6 |
Forward: 5′‐GGCCAGGCCGTTCATCTGGT‐3′ Reverse: 3′‐TCCATTCTGCATCACGCTGT‐5′ |
T. pseudospiralis | 158 |
| 7 |
Forward: 5′‐ATCGCGCTCTCGCGATTGGG‐3′ Reverse: 3′‐TCCATTCTGCATCACGCTGT‐5′ |
T. pseudospiralis | 103 |
| 8 |
Forward: 5′‐TCCCAGACCAGCGGNAGCAC‐3′ Reverse: 3′‐TCCATTCTGCATCACGCTGT‐5′ |
T. nativa | 372 |
| 9 |
Forward: 5′‐GACCCAGCGCGCTTTCGTTG‐3′ Reverse: 3′‐TCCATTCTGCATCACGCTGT‐5′ |
T. nativa | 317 |
| 10 |
Forward: 5′‐CGGATACCACGGGCCGATGT‐3′ Reverse: 3′‐TCCATTCTGCATCACGCTGT‐5′ |
T. nativa | 226 |
| 11 |
Forward: 5′‐AGTCGCCCAGCTTGATCGCG‐3′ Reverse: 3′‐TCCATTCTGCATCACGCTGT‐5′ |
T. nativa | 116 |
| 12 |
Forward: 5′‐ATCGCGCTCTCGCGATTGGG‐3′ Reverse: 3′‐CAACCGATACCGAACGGACC‐5′ |
T. pseudospiralis | 116 |
| 13 |
Forward: 5′‐TCCCAGACCAGCGGNAGCAC‐3′ Reverse: 3′‐CGGACCGAACTCTGGTACAG‐5′ |
T. nativa | 319 |
| 14 |
Forward: 5′‐CGGATACCACGGGCCGATGT‐3′ Reverse: 3′‐CGGACCGAACTCTGGTACAG‐5′ |
T. nativa | 171 |
| 15 |
Forward: 5′‐TCCCAGACCAGCGGNAGCAC‐3′ Reverse: 3′‐ACATCGGCCCGTGGTATCCG‐5′ |
T. nativa | 168 |
| 16 |
Forward: 5′‐TCCCAGACCAGCGGNAGCAC‐3′ Reverse: 3′‐GTGAGTCCAGCAGCAACCCG‐5′ |
T. nativa | 129 |