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. 2016 Mar;44(3):352–355. doi: 10.1124/dmd.115.068437

Fig. 2.

Fig. 2.

Effect of PPAPDC2 knockdown on SQ1-mediated CAR activation in primary cultured rat hepatocytes. (A) Validation of rPPAPDC2 knockdown. Twenty-four hours after plating, primary cultured rat hepatocytes were transiently transfected with a validation reporter vector for rat PPAPDC2 (rPPAPDC2-Luc) and either a nontargeting control shRNA (shNT) or a shRNA targeting rat PPAPDC2 (shrPPAPDC2). After 48 hours, hepatocytes were harvested for measurement of luciferase activity. Each bar represents the mean ± S.E.M. of mean normalized (firefly/Renilla) luciferase measurements from three independent experiments (n = 3). (B) Rat hepatocytes were transiently transfected with the CYP2B1-Luc reporter and either the shNT or shrPPAPDC2 plasmid. Then, 24 hours after transfection, cultures were incubated for 48 hours in medium alone (UT) or containing 0.1 μM SQ1 and harvested for measurement of luciferase activity. Each bar represents the mean ± S.E.M. of mean normalized (firefly/Renilla) luciferase measurements from three independent experiments (n = 3). All values are normalized to the shNT/UT group. *P < 0.05 (significantly different than the shNT/SQ1 group).