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. 2016 Mar;89(3):323–334. doi: 10.1124/mol.115.101758

Fig. 2.

Fig. 2.

Enhancement of endogenous Kv7.5 current in A7r5 cells by elevation of intracellular [cAMP]. (A) Current-voltage (I-V) relationships of endogenous Kv7.5 currents recorded in A7r5 cells before (control, solid circles), after 15 minutes of treatment with 1 mM 8-bromo cAMP (8Br-cAMP) (open circles), and after 10 minutes of washout (solid triangles). Currents were normalized to currents recorded at −20 mV before application of 8Br-cAMP [n = 4, * indicates significant difference from control, P < 0.05, one-way repeated-measures analysis of variance (ANOVA)]. (B) I-V relationships of endogenous Kv7.5 currents recorded in A7r5 cells before (control, solid circles), after 5 minutes of treatment with 1 μM forskolin (open circles), and after 10 minutes of washout (solid triangles). Currents were normalized to currents recorded at −20 mV before application of forskolin (n = 6, * indicates significant difference from control, P < 0.05, one-way repeated-measures ANOVA). (C) I-V relationships of endogenous Kv7.5 currents recorded in A7r5 cells before (control, solid circles), after 5 minutes of treatment with 1 μM forskolin in the presence of 500 μM IBMX (open circles), and after 10 minutes of washout (solid triangles). Currents were normalized to currents recorded at −20 mV before application of forskolin/IBMX (n = 4, * indicates significant difference from control, P < 0.05, one-way repeated-measures ANOVA). (D) I-V relationships of endogenous Kv7.5 currents recorded in A7r5 cells before (control, solid circles) and after treatment with papaverine (10 μM open circles, 100 μM open triangles). Currents were normalized to currents recorded at −20 mV before application of papaverine (n = 4, * indicates significant difference from control, P < 0.01, one-way repeated-measures ANOVA).