Skip to main content
. 2016 Mar;89(3):323–334. doi: 10.1124/mol.115.101758

Fig. 7.

Fig. 7.

Activation of endogenous βARs in A7r5 cells enhances PKA-dependent phosphorylation of exogenous hKv7.5 but not hKv7.4 channels. (A) Representative images of A7r5 cells exogenously expressing hKv7.5 channels (top panels) and hKv7.4 channels (bottom panels); untreated (control), treated with 1 μM isoproterenol (ISO) for 5 minutes, and treated with 1 μM ISO for 5 minutes after pretreatment with 1 μM KT5720 for 30 minutes (ISO + KT). PLAs were conducted on hKv7.5-expressing cells labeled with a combination of mouse anti-FLAG antibody and rabbit anti-phospho-Ser/Thr PKA substrate antibody or on hKv7.4-expressing cells labeled with a combination of mouse anti-KCNQ4 antibody and rabbit anti-phospho-Ser/Thr PKA substrate antibody. (B and C) Bar graphs summarizing the number of PLA signals/cell in A7r5 cells exogenously expressing hKv7.5 (B) and hKv7.4 (C) under control conditions (c), after 30 minutes of treatment with 1 μM KT5720 (c + KT), after 5 minutes of treatment with 1 μM ISO in the absence (ISO) or presence of pretreatment with 1 μM KT5720 (ISO + KT), and in cells where the primary antibodies were omitted (−1°ab); * and #, P < 0.001 from all group analyses of variance on ranks, n = 12–21.