TABLE 1.
Effect of 1 μM isoproterenol on the voltage dependence of endogenous Kv7.5 and exogenous homomeric hKv7.4, homomeric hKv7.5, and heteromeric hKv7.4/7.5 channels
| Kv7 isoform |
Gmax ISO/Gmax Control x 100% |
V0.5 |
k |
||
|---|---|---|---|---|---|
| Control |
1 μM ISO |
Control |
1 μM ISO |
||
| % | mV | mV | mV | mV | |
| Endogenous Kv7.5, n = 10 | 259.3 ± 33.8a | −48.5 ± 2.5 | −47.8 ± 2.7 | 9.5 ± 1.0 | 11.3 ± 0.8 |
| Exogenous hKv7.5, n = 5 | 267.4 ± 16.6b | −46.2 ± 2.5 | −46.1 ± 2.1 | 12.3 ± 0.8 | 13.6 ± 1.7 |
| Exogenous hKv7.4, n = 5 | 98.1 ± 3.1 | −28.6 ± 2.0 | −28.2 ± 1.6 | 10.8 ± 0.7 | 10.7 ± 0.7 |
| Exogenous hKv7.4/7.5, n = 5 | 134.9 ± 9.3a | −34.0 ± 3.9 | −35.5 ± 5.6 | 11.3 ± 0.8 | 12.1 ± 0.9 |
Gmax, maximal conductance; ISO, isoproterenol; k, slope factor; V0.5, voltage of half-maximal activation.
a
Significantly different from control, paired Student’s t test, P < 0.05.
b
Significantly different from control, paired Student’s t test, P < 0.001.