Table 3.
Sensitivity, specificity, and accuracy of the qPCR assays analyzed. Host‐specific Bacteroides qPCRs developed in this study: HMprobeBac (human), PLprobeBac (poultry), PGprobeBac (pig), CWprobeBac (cow), and Bacprobe (total Bacteroides). Host‐specific Bacteroides qPCRs developed by other authors and assayed in this study: HF183 (human), Pig‐2‐Bac (pig), and Rum‐2‐Bac (ruminants)
| Value | Host‐specific Bacteroides qPCRs in this study | Other proposed host‐specific targets | ||||||
|---|---|---|---|---|---|---|---|---|
| HMprobeBac | PLprobeBac | PGprobeBac | CWprobeBac | Bacprobe | HF183 | Pig‐2‐Bac | Rum‐2‐Bac | |
| Sensitivity | 0.45 | 0.73 | 0.50 | 1.00 | 1.00 | 1.00 | 1.00 | 0.70 |
| Specificity | 1.00 | 1.00 | 0.97 | 0.00 | 1.00 | 0.20 | 0.50 | 1.00 |
| Accuracy | 0.86 | 0.93 | 0.84 | 0.25 | 1.00 | 0.38 | 0.65 | 0.93 |