Figure 1.

Changes in Corynebacterium glutamicum protein acetylation and succinylation during glutamate overproduction. C. glutamicum ATCC13869 cells were grown in glutamate production medium, and glutamate production was induced by adding Tween 40 after 3 h of cultivation; cells were harvested at the indicated times. (A) Growth curves (left) and concentrations of glucose and glutamate in the filtered culture medium (right). Open and closed symbols represent nonproducing and glutamate‐producing conditions, respectively. (B) Western blot analysis using anti‐acetyl lysine (left) or anti‐succinyl lysine (right) antibody. Lysate aliquots containing 25 μg of protein were separated by 8% SDS‐PAGE and subjected to western blot analysis. N, nonglutamate‐producing conditions; P, glutamate‐producing conditions.