Schematic representation of lysine acetylation and succinylation in central carbon metabolism in the nonproducing (left) and glutamate‐producing (right) conditions. Red, blue, and green circles represent acetylation, succinylation, and overlapping sites, respectively. Circles at corresponding positions represent an identical residue, located in order from the N to C‐terminus. Pgi, glucose‐6‐phosphate isomerase (NCgl0817); Pfk, 6‐phosphofructokinase (NCgl1202); GlpX, fructose 1,6‐bisphosphatase II (NCgl0976); Fba, fructose‐bisphosphate aldolase (NCgl2673); TpiA, triosephosphate isomerase (NCgl1524); Gap, glyceraldehyde‐3‐phosphate dehydrogenase (NCgl1526); GapX, glyceraldehyde‐3‐phosphate dehydrogenase (NCgl0900); Pgk, phosphoglycerate kinase (NCgl1525); GpmA, phosphoglyceromutase (NCgl0390); Eno, enolase (NCgl0935); Pyk, pyruvate kinase (NCgl2008); Zwf, glucose‐6‐phosphate 1‐dehydrogenase (NCgl1514); DevB, 6‐phosphogluconolactonase (NCgl1516); Gnd, 6‐phosphogluconate dehydrogenase (NCgl1396); Rpi, ribose‐5‐phosphate isomerase B (NCgl2337); Tkt, transketolase (NCgl1512); Tal, transaldolase (NCgl1513); AceE, pyruvate dehydrogenase subunit E1 (NCgl2167); GltA, type II citrate synthase (NCgl0795); Acn, aconitate hydratase (NCgl1482); Icd, monomeric isocitrate dehydrogenase (NADP+) (NCgl0634); Kgd, alpha‐ketoglutarate decarboxylase (NCgl1084); AceF, dihydrolipoamide acetyltransferase (NCgl2126); Lpd, dihydrolipoamide dehydrogenase (NCgl0355); SdhA, succinate dehydrogenase flavoprotein subunit (NCgl0360); SdhB, succinate dehydrogenase iron‐sulfur subunit (NCgl0361); FumC, fumarate hydratase (NCgl0967); Mdh, malate dehydrogenase (NCgl2297); Mqo, malate:quinone oxidoreductase (NCgl1926); AceA, isocitrate lyase (NCgl2248); GlcB, malate synthase G (NCgl2247); Pta, phosphotransacetylase (NCgl2657); AckA, acetate kinase (NCgl2656); PoxB, pyruvate dehydrogenase (NCgl2521), Pyc, pyruvate carboxylase (NCgl0659); Ppc, phosphoenolpyruvate carboxylase (NCgl1523); PckG, phosphoenolpyruvate carboxykinase (NCgl2765); Gdh, glutamate dehydrogenase (NCgl1999). Detailed information is shown in Table S4.